Girke-girke sodium hyaluronate gel don tiyata

Ƙirƙirar yanzu tana da alaƙa da haɗin giciye sodium hyaluronate gel don filler nama don tiyatar filastik da hanyar shirye-shiryensa.Maganin alkaline na sodium hyaluronate yana amsawa tare da dogon sarkar alkane mai ɗauke da rukunin epoxy da wakilin haɗin giciye mai ɗauke da rukunin epoxy na tsawon awanni 2˜5 a 35 ° C.˜50 ° C. don samar da hyaluronate mai haɗe-haɗe, sannan a wanke. gelled da haifuwa, don shirya gel.Daga cikin abin da, molar rabo na sodium hyaluronate: giciye-linking wakili dauke da epoxy group: dogon sarkar alkane dauke da epoxy rukuni ne 10:4˜1:1˜4;adadin carbon atom na wannan dogon sarkar alkane dauke da epoxy kungiyar ne 6 zuwa 18. Gel da aka shirya a cikin abin da aka kirkira na iya, a daya hannun, yadda ya kamata inganta juriya ga enzymolysis ya zama mafi barga, da kuma a daya hannun, kula. Kyakkyawan bioacompatibility na sodium hyaluronate ba tare da shafar allurar sa ba.

Crosslinked sodium hyaluronate gel don tiyata filastik tiyata da hanyar shirye-shiryensa:

Ƙirƙirar tana da alaƙa da hanyar shirye-shiryen sodium hyaluronate gel mai haɗe-haɗe don aikin tiyata na filastik, wanda ke da alaƙa ta ƙunshi matakai masu zuwa:
(1) Sodium hyaluronate busassun foda an tarwatsa a cikin 10?wt%～20?Maganin gauraye wanda ya ƙunshi wt% sodium hydroxide aqueous bayani da acetone aka yi amfani da shi don samun sodium hyaluronate asali dakatar, sa'an nan crosslinking wakili 1,4?Butanediol diglycidyl ether BDDE yana haɗe-haɗe-haɗe-haɗe don samun kayan haɓaka, don fara amsawa don samar da hyaluronate sodium mai haɗin giciye;A karkashin yanayin motsawa, an gama amsawa bayan an kiyaye abun da aka dauki a 35 ℃ ~ 50 ℃ don 5 ~ 8 hours, da ƙimar pH na kayan hadewar ruwa mai ƙarfi bayan an daidaita halayen zuwa 7 tare da maida hankali hydrochloric acid;A cikin abin da maida hankali na sodium hyaluronate a cikin abun da ke faruwa shine 2?Wt% ~ 5wt%, yawan ma'auni na ma'auni na crosslinking zuwa sodium hyaluronate shine (1: 1.3) ~ (1: 1.8);
(2) Tace cakuda mai ƙarfi da ruwa na pH = 7 bayan amsawar cire ruwa.Sauran kayan ana wanke su da acetone zuwa GC?MS don gano abun ciki na BDDE ƙasa da 2ppm.Sauran kayan sun hada da farin foda da gel mai haske, sannan busassun kayan ana busar da su don samun farin busasshen busasshen ruwa wanda ba zai iya narkewa ba, wato, giciye sodium hyaluronate foda.
(3) Sieving da rabuwa da crosslinked sodium hyaluronate foda samu ta injin bushewa a mataki ② da kuma tattara da sieved foda;
(4) Ƙara ruwan da aka yi da ruwa zuwa foda mai tsabta wanda aka tattara a mataki na 3, don haka sodium hyaluronate foda mai haɗin giciye ya cika kuma an tsarkake shi don 6 ~ 10 hours a dakin da zafin jiki daga 15 zuwa 35 digiri, kuma an tattara sassan gel zuwa ga samu crosslinked sodium hyaluronate gel.
(5) An ƙara buffer isotonic PBS zuwa gel da aka tattara a mataki na 4 kuma an tsarkake shi don 6 ~ 10 hours a dakin da zafin jiki daga 15 zuwa 35 digiri.Bayan tacewa, an cire PBS kuma an tattara gel.An binne gel ɗin ta hanyar yin amfani da ƙayyadaddun farko da ƙayyadaddun allo na biyu.An samo nau'ikan gels daban-daban guda 3, kuma gels 3 an haifuwa kuma an cika su a cikin sirinji da za a iya zubarwa.